Construction of Reverse Genetics System for Feline Calicivirus FCV-BJ616 and Proteomic Analysis.

ABSTRACT: Feline calicivirus (FCV) is a primary cause of upper respiratory tract infections and oral ulcerative disease in cats and exhibits substantial genetic diversity that complicates prevention and control. In this study, we isolated the FCV-BJ616 strain, established a reverse-genetics system, and investigated its pathogenic mechanisms, thereby providing a foundation for antibody-based therapies and broad-spectrum vaccine development. The virus was purified by three rounds of plaque cloning, and its morphology was examined by electron microscopy. VP1 expression was confirmed by immunofluorescence and Western blotting. Using integrated systems-biology and reverse-genetics approaches, an infectious clone of rFCV-BJ616 was successfully assembled and rescued, exhibiting genetic stability comparable

SUBMITTER: Xu C 

PROVIDER: S-EPMC12849204 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature