Molecular architecture of mesoderm cells across early to middle stage of human embryo development at single-cell resolution.

ABSTRACT:

Background

The differentiation of mesodermal cells (MCs) in the early stage of embryonic development contributes to the organogenesis of several core organs. However, the single-cell molecular architecture of MCs and the key molecular events during the differentiation remain unclear.

Methods

We performed single-cell RNA sequencing (RNA sequencing) and single-cell assay for transposase-accessible chromatin (ATAC-Seq) to analyze the developmental features of MCs to heart, kidney, spleen, liver, and brain in human embryos at gestational ages 7-17 weeks.

Results

We found that EGR1 might be relevant to the differentiation of heterogeneous MC sub-clusters. Meanwhile, RPL10P9⁺PTMAP5⁺ MCs had the closest expression profiling with endocardial cells. NDUFA

SUBMITTER: Zhang W 

PROVIDER: S-EPMC12849250 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature