Strand-specific detection of cell-associated sense and antisense HIV-1 RNAs in splenocytes and PBMC from PLWH.

ABSTRACT:

Background

Variation in the level of cell-associated HIV-1 RNA is an important parameter followed in clinical trials focusing on HIV-1 infection, latency or reactivation. In addition to sense products, HIV-1 also expresses antisense products that can modulate HIV-1 replication, either positively through the antisense protein ASP or negatively through repressive noncoding antisense RNAs. Therefore, quantification of both sense and antisense HIV-1 products could provide key information for monitoring the dynamics of viral replication in vivo. While the ASP protein is difficult to detect even in vitro, antisense RNAs can be detected both in vitro and in vivo. The aim of this study was therefore to establish protocols for the specific quantification of sense and antisense transcription

SUBMITTER: Waast L 

PROVIDER: S-EPMC12849480 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature