Reactive oxygen species-associated molecular landscape uncovers diagnostic and immune features in osteoarthritis.

ABSTRACT:

Background

Osteoarthritis (OA) is a prevalent degenerative joint disease characterized by joint inflammation and degradation. Reactive oxygen species (ROS) are implicated in the pathogenesis of OA, yet the molecular pathways linking ROS-related genes to the immune microenvironment remain poorly understood.

Methods

We integrated publicly available OA gene expression datasets and performed batch correction. ROS-related pathways were curated from the MSigDB, and pathway activity was assessed using ssGSEA. We identified differentially expressed genes (DEGs) in both training and validation cohorts, followed by GO and KEGG enrichment analyses. An elastic net regression model was constructed to calculate a risk score based on critical ROS-related DEGs. Model performance was evaluat

SUBMITTER: Zhou J 

PROVIDER: S-EPMC12849670 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature