Targeted chondrogenic differentiation of human MSCs using niosomes for SOX9 gene delivery: comparison of minicircle and conventional plasmids.

ABSTRACT:

Background

Niosomes represent a promising non-viral gene delivery system, offering an alternative to viral vectors for the genetic modification of hard-to-transfect cells, such as mesenchymal stem cells (MSCs), which are pivotal in regenerative medicine. Specifically, SOX9 gene transfer is a valuable strategy for cartilage tissue repair, as it promotes chondrocyte differentiation while repressing hypertrophic and osteogenic markers. In this study, we investigated the potential of niosomes to deliver SOX9, using both parental and minicircle plasmids, to induce chondrogenic differentiation in primary bone marrow-derived human MSCs (hMSCs).

Methods

Niosomes were synthesised using the thin-film hydration method and complexed with either parental or minicircle SOX9 plasmids to fo

SUBMITTER: Lopez-Seijas J 

PROVIDER: S-EPMC12849684 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature