Thiophene Esters Are Not Selective for H₂S and Undergo Thiol and Esterase Cleavage.

ABSTRACT: Hydrogen sulfide (H₂S) fluorescent probes are important tools for imaging and understanding H₂S in biology. One significant requirement for such probes is that they are highly selective for H₂S over competing analytes, which are often present at much higher levels than endogenous H₂S. Different approaches have been used to generate selective H₂S probes, and recently, highly selective probes using 2-thiophene esters have been reported. We report here that in contrast to prior reports, thiophene ester probes are not selective for H₂S but rather report on both biothiols and esterase activity. We do demonstrate, however, that the rate of reactivity toward H₂S can be enhanced by incorporating an ortho aldehyde,

SUBMITTER: Smith HM 

PROVIDER: S-EPMC12849719 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature