Follicular fluid extracellular vesicles improve bovine oocyte quality via lipid and mitochondrial modulation.

ABSTRACT: In vitro maturation (IVM) systems remain sub-optimal, compromising oocyte cytoplasmic competence and subsequent embryo quality by failing to reproduce follicular fluid (FF) microenvironment communication, specifically by lacking extracellular vesicles (ffEVs). In order to determine the contribution of ffEVs on oocyte maturation and quality, oocytes were assessed for nuclear maturation, mitochondrial activity, reactive oxygen species (ROS) levels, and lipid metabolism parameters such as lipid droplet (LD) area, HSL phosphorylation and glycerol release levels, after IVM of COC with or without ffEVs. Subsequent embryo development rates and blastocyst quality (total cell number, mitochondrial activity and LD area) were also evaluated. Additionally, the relative abundance of mRNA and miR

SUBMITTER: Schneberger F 

PROVIDER: S-EPMC12849768 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature