Modulation of Aerobic Glycolysis Genes During the Progression of Retinitis Pigmentosa.

ABSTRACT:

Purpose

Photoreceptors are retinal cells with a high glucose metabolism and retinal degeneration, specifically retinitis pigmentosa (RP), affects glycolysis. We aimed to evaluate changes in the expression of genes related to glucose metabolism in rod photoreceptors at different stages of retinal degeneration in murine models and human retinal organoids.

Methods

RNA sequencing (RNA-seq) analysis was performed on a photoreceptor-like cell line induced to undergo degeneration and validated by real-time qPCR analysis of retinas from two murine models and one human organoid model of RP. Bioinformatic analysis was performed on published RNA-seq datasets from three murine RP models. Real-time qPCR analysis was also performed on retinas treated with an adeno-associated virus type 2

SUBMITTER: Adani E 

PROVIDER: S-EPMC12849827 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature