Enriching Higher Affinity Aptamers by Addressing the Kinetic Aspect of the DNA Strand-Displacement Reaction.

ABSTRACT: Selection of high affinity aptamers is the basis for aptamer-related applications. Most aptamer selections prioritize thermodynamic factors such as the target concentration but neglect interaction kinetics. Using ampicillin as a target, aptamer selections were carried out based on the DNA strand-displacement reaction. A low-affinity aptamer (K_d = 12.7 µM) dominated under the typical condition of gravity flow with a short interaction time of 1 to 2 min, whereas a high affinity aptamer (K_d = 1.8 µM) dominated the library with an additional 10 min incubation. These two aptamers differ only in three positions, and the strand-displacement kinetics was determined to be responsible for the selection outcomes. Kinetic effects were also observed in aptamer selection for adenos

SUBMITTER: Ding Y 

PROVIDER: S-EPMC12850998 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature