Targeting Oxidative Stress and Apoptosis via PI3K/Akt/Nrf2 Pathway: The Therapeutic Role of Bletilla striata Polysaccharide in Diabetic Wound Repair.

ABSTRACT:

Background

Diabetic wounds are challenging and lack efficient therapeutic solutions. Bletilla striata polysaccharide (BSP) has garnered interest for its bioactivity and antioxidant ability in wound healing. This research explores the mechanisms through which BSP alleviates oxidative stress (OS) in L929 cells and prevents cell apoptosis under high-glucose (HG) conditions. Furthermore, the research evaluates its promise as a novel therapeutic approach for facilitating recovery in diabetic wounds.

Methods

Various doses of glucose and BSP were administered to L929 cells to evaluate their effects on cell viability, OS, activation of the PI3K/Akt/Nrf2 signaling pathway, and apoptosis. These effects were assessed using CCK-8 assays, commercial kits, and western blots (WBs).

SUBMITTER: Xu S 

PROVIDER: S-EPMC12851404 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature