Characterization of the Tychonamide Biosynthetic Pathway in the Recently Described Cyanobacterial Species Floridanema aerugineum.

ABSTRACT: Secondary metabolite profiling of a recently described new cyanobacterial species Floridanema aerugineum identified abundant amounts of the polyketide-peptide tychonamide A. Investigation of the biosynthetic gene clusters in the F. aerugineum genome identified a putative tychonamide biosynthetic gene cluster with module and domain architecture consistent with the tychonamide A structure. High resolution liquid chromatography-mass spectrometry/MS (LC-MS/MS) identified new tychonamide analogs C-E (1-3), and bioactivity predictions led us to test the protease inhibition activities of the tychonamides. The results of this analysis illustrated the biosynthesis of the 3-amino-2,5,7-trihydroxy-8-phenyloctanoic acid moiety (Atpoa) in tychonamide A, further deduced the absolute

SUBMITTER: Chopade A 

PROVIDER: S-EPMC12851428 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature