Neutrophil-derived extracellular vesicles in the plasma of alpha-1 antitrypsin deficient individuals reveal pro-inflammatory metabolic and transcriptomic signatures.

ABSTRACT: Alpha-1 antitrypsin deficiency (AATD) is a genetic disorder caused by mutations in SERPINA1, leading to chronic obstructive pulmonary disease (COPD) and liver disease. Neutrophils are key regulators of inflammatory signaling networks; however, their dysregulation in AATD and the underlying molecular mechanisms remain poorly understood. Here, we employed a multi-omics approach integrating RNA sequencing (RNA-seq) and metabolomics to comprehensively characterize neutrophil dysfunction in AATD. RNA-seq analysis of blood neutrophils from AATD individuals revealed transcriptional dysregulation in genes involved in intracellular signaling, immune response regulation, and metabolic adaptation. Isolation and characterization of neutrophil-derived extracellular vesicles (EV) demonstrated an

SUBMITTER: Greenberg ZF 

PROVIDER: S-EPMC12851533 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature