Identifying regulators of associative learning using a protein-labelling approach in Caenorhabditis elegans.

ABSTRACT: The ability to learn and form memories is critical for animals to make choices that promote their survival. The biological processes underlying learning and memory are mediated by a variety of genes in the nervous system, acting at specific times during memory encoding, consolidation, and retrieval. Many studies have utilised candidate gene approaches or random mutagenesis screens in model animals to explore the key molecular drivers for learning and memory. We propose a complementary approach to identify this network of learning regulators: the proximity-labelling tool TurboID, which promiscuously biotinylates neighbouring proteins, to snapshot the proteomic profile of neurons during learning. To do this, we expressed the TurboID enzyme in the entire nervous system of Caenorhabditis el

SUBMITTER: Rahmani A 

PROVIDER: S-EPMC12851583 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature