Portable Dynamic Laser Speckle Imaging for Rapid Antimicrobial Susceptibility Testing.

ABSTRACT: Antimicrobial resistance remains a pressing global health threat. Conventional antimicrobial susceptibility testing (AST) methods are limited by long incubation times and centralized laboratory requirements, hindering timely decision-making in resource-limited settings. This study introduces a 3D printed, portable dynamic laser speckle imaging (pDLSI) device for rapid AST. This device has a 10 × 3 × 3 cm³ footprint, equipped with a low-cost laser diode, a lens assembly, and a cuvette sample holder. Speckle fluctuations induced by bacterial activity are captured on a cellphone camera, visualized using spatiotemporal decorrelation maps, and analyzed using machine learning to determine minimum inhibitory concentrations within 2-3 h. To demonstrate the performance, we studied two re

SUBMITTER: Yang J 

PROVIDER: S-EPMC12851625 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature