Instrumental Dispersion Evaluation on Supercritical Fluid Chromatography-Medium Vacuum Chemical Ionization Tandem Mass Spectrometry.

ABSTRACT: Instrumental dispersion in the ion source can severely distort fast chromatographic peaks in supercritical fluid chromatography (SFC)-mass spectrometry (MS). Despite this importance, the dispersion characteristics specific to medium-vacuum chemical ionization (MVCI) sources have not been quantitatively investigated. In this work, we combine targeted experiments with established computational tools-computational fluid dynamics (CFD) and electrostatic field simulation-to characterize ion transport in the MVCI flow tube. Arrival profiles of vitamin K₁ (VK₁) ions monitored by selected ion monitoring consistently showed a reproducible two-component structure consisting of an early narrow bandwidth ion packet followed by a delayed shoulder. CFD calculations reproduce this t

SUBMITTER: Hondo T 

PROVIDER: S-EPMC12851858 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature