Gnotobiotic growth and phosphorus limitation of Arabidopsis thaliana and co-occurring microbes on phosphated iron oxides.

ABSTRACT: The macronutrient phosphorus is vital for sustaining cellular processes in all life forms. Due to its frequent adsorption on iron minerals, phosphorus bioavailability is low in many soils. While the abiotic adsorption of phosphate on iron minerals has been well studied, the direct effects of this process on bioavailability to plants and microbes has not been thoroughly investigated in a simplified laboratory system. We developed a hydroponic growth system that uses hydrous ferric oxide (HFO) to induce phosphorus limitation and can enable both plant and microbial cultivation as well as gnotobiotic co-culture. We demonstrate that this system can be used for phosphorus-limited growth of the model plant Arabidopsis thaliana as well as two root-associated bacterial isolates (from the genera Rhi

SUBMITTER: Mackie AM 

PROVIDER: S-EPMC12852198 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature