Annexin A2 and lamin B join membrane recycling compartments for the assembly of biomolecular condensates operating in mitotic partitioning.

ABSTRACT: Localization of the actin-, lipid- and mRNA-binding protein Annexin A2 (AnxA2) in dividing cells revealed its presence in large spherical structures which are confined to the cell periphery and frequently co-align with astral microtubules. These structures appear during prometaphase and disappear at telophase, coinciding with the mitotic breakdown and subsequent reformation of the nuclear lamina and envelope. Their size increases as cells progress to anaphase, while their number decreases, suggesting that they are capable of fusion. Treatment of cells with the aliphatic alcohol propylene glycol led to rapid and reversible disassembly of the structures, providing further evidence that they correspond to biomolecular condensates. Notably, the condensates enclose compartments involved in bios

SUBMITTER: Grindheim AK 

PROVIDER: S-EPMC12852427 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature