Early and progressive retinal microglial changes in APP^NL-F/NL-F mouse model of Alzheimer's disease revealed by an automated image analysis software.

ABSTRACT: Alzheimer's disease (AD) is characterized by the accumulation of misfolded proteins that trigger neuroinflammation and neuronal loss. The retina, as an extension of the central nervous system, mirrors these pathological processes and represents a potential biomarker. Microglial activation, a key component of neuroinflammation, can be morphologically assessed through automated image analysis. This study performed a quantitative and morphological analysis of retinal microglia in the APP^NL-F/NL-F mouse model of AD across aging (6-20 months) and comparing them with age-matched C57BL/6 J controls using an automated image analysis software. A cross-sectional design was applied to 72 mice (36 APP^NL-F/NL-F and 36 WT). Retinas samples were processed by Iba-1 immunohistochemist

SUBMITTER: Sanchez-Puebla L 

PROVIDER: S-EPMC12852431 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature