A synthetic biology toolkit for rationally designing genetic circuits in <i>Acinetobacter baumannii</i>.

ABSTRACT:

Introduction

Antimicrobial resistance (AMR) poses a severe global health threat, with Acinetobacter baumannii among the critical AMR priorities highlighted by World Health Organization (WHO). This Gram-negative pathogen exhibits intrinsic resistance traits, exceptional environmental persistence, and high genomic plasticity, harboring resistance islands.

Methods

To combat AMR through synthetic biology, this study characterizes a library of BioBrick parts to be adopted in A. baumannii engineering and develops a modular CRISPR interference (CRISPRi) platform.

Results

Key components were characterized, including two plasmid vectors, a library of inducible and constitutive promoters, and a CRISPRi-mediated repression system; for the latter, a testbed for biof

SUBMITTER: Letrari S 

PROVIDER: S-EPMC12852451 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature