ClC-2 Contributes to Hypotonicity-Induced Adrenal Aldosterone Secretion.

ABSTRACT:

Aim

The zona glomerulosa (ZG) of the adrenal cortex regulates blood pressure and electrolyte homeostasis through aldosterone production. In ZG cells, potassium and angiotensin II (Ang II) trigger calcium oscillations that drive aldosterone synthesis. Changes in serum osmolality also modulate aldosterone production in a chloride-dependent fashion, but the involved proteins remain unclear. Because the chloride channel ClC-2 is activated by hypoosmolality, we investigated its role in ZG osmoregulation.

Methods

We used Clcn2 knockout (KO) and wild-type (WT) mice. Explanted adrenal glands were incubated with iso- and hypotonic solutions for measurements of aldosterone. Acute adrenal slices were studied using calcium and chloride sensitive fluorescent dyes. We also investigated Cl

SUBMITTER: Volkert M 

PROVIDER: S-EPMC12852535 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature