The Rac1-USP11 feedback amplification loop: a radiation-activated engine driving radioresistance in hepatocellular carcinoma.

ABSTRACT:

Background

Radioresistance is an objective biological factor that affects the efficacy of clinical radiotherapy in hepatocellular carcinoma (HCC). However, the mechanism involved has not yet been fully understood.

Methods

Integrative analysis of HCC patient data with RNA-seq and IHC. Radiosensitivity assessed by colony formation assays using HCC cell lines and xenograft models established in B-NDG mice. USP11 promoter activity measured by dual luciferase reporter. Protein interactions analyzed by Co-IP/GST pulldown; post-translational modifications by ubiquitylation assays. Rac1 activity was quantified by measuring GTP-bound levels. Rac1 structural dynamics simulated through molecular dynamics. Functional validation performed using pharmacological inhibitors, genetic depleti

SUBMITTER: Zhou K 

PROVIDER: S-EPMC12852764 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature