Vascularized human brain organoids as a model of the brain-peripheral axis in HIV-1 neuropathogenesis.

ABSTRACT: Advances in stem cell biology and tissue engineering have enabled the generation of brain organoids from human induced pluripotent stem cells (hiPSCs), providing powerful platforms to model human brain development and neurodegenerative diseases. However, the lack of vascularization in conventional brain organoids limits nutrient and oxygen diffusion, thereby constraining their viability and physiological relevance. To overcome this limitation, we developed vascularized brain organoids using both normal hiPSCs and hiPSCs overexpressing the endothelial transcription factor ETS variant 2 (ETV2). Furthermore, this model also provides a unique opportunity to investigate how HIV-1 disrupts endothelial integrity within the CNS. Organoids were generated through co-culture differentiation using a m

SUBMITTER: Selvam S 

PROVIDER: S-EPMC12852793 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature