In silico design and immunoinformatics assessment of a multiepitope vaccine targeting borealpox virus.

ABSTRACT: Borealpox virus is an emerging zoonotic pathogen with rising global prevalence and no approved vaccines or targeted treatments. This study aimed to design a multi-epitope subunit vaccine against Borealpox virus. The viral cell-surface binding protein was selected as the primary antigenic target due to its role in host-cell attachment. The finalized vaccine construct comprises 163 amino acids and includes β-defensin 3 as an adjuvant, a PADRE sequence, and suitable linkers to enhance immunogenicity. Structural prediction revealed a stable conformation, with 97.4% of residues located in the most favored regions of the Ramachandran plot. The vaccine exhibited a VaxiJen antigenicity score of 0.7089 and was predicted to be non-allergenic. Population coverage analysis indicated that the selected

SUBMITTER: Naveed M 

PROVIDER: S-EPMC12852795 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature