In vitro evaluation of Bacillus subtilis isolated from Apis mellifera for potential use as a probiotic feed additive in honeybees.

ABSTRACT: The honeybee Apis mellifera plays a crucial role in global agriculture but faces significant threats from pathogens, pesticides, and nutritional stress in the beekeeping industry. In this study, we evaluate the potential application of Bacillus subtilis MBLB2796 isolated from the honeybee gut. Solid-state fermentation of soybean meal with MBLB2796 reduced allergenic proteins and anti-nutritional factors, enhancing its nutritional quality. Strain MBLB2796 also exhibited high hydrolytic enzyme activities (15.5 mm of protease, 13.5 mm of amylase, and 15.0 mm of cellulase), strong antimicrobial activity (20.5 mm) against Paenibacillus larvae, and antioxidant activity against DPPH and ABTS radicals (66.2% and 62.3%, respectively). Strain MBLB2796 demonstrated a strong survival rate after freeze

SUBMITTER: Hwang CY 

PROVIDER: S-EPMC12852938 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature