Establishment and Characterization of MCA23, a Novel Mouse Intrahepatic Cholangiocarcinoma Cell Line.

ABSTRACT:

Introduction

Intrahepatic cholangiocarcinoma (ICC) is an aggressive type of malignancy. Recent advancements have highlighted the importance of the tumor immune microenvironment in therapeutic responses and prognosis. However, the lack of a mouse-derived ICC cell line and current mouse models limit explorations of the TME in ICC. Therefore, establishing suitable preclinical models is critical.

Methods

In the present study, an ICC mouse model was established using hydrodynamic transfection. Primary ICC cells were isolated, purified, and cultured in the liver tissues of these mice. Cellular behaviors, molecular characterization, and genetic profiles were evaluated in vitro. The tumorigenic and metastatic potential of the cells was determined in vivo. Drug sensitivity was tested

SUBMITTER: He Y 

PROVIDER: S-EPMC12853219 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature