Analytical Comparison of Three Commercial Lysing Reagents for Full-Spectrum Flow Cytometric Immunophenotyping.

ABSTRACT:

Introduction

Flow cytometry (FC) enables rapid identification of cell lineages based on their fluorescent and light-scattering properties, playing a critical role in diagnosing and monitoring oncohematological diseases. Standardization of procedures in spectral FC is essential for method validation studies.

Methods

We designed a comparative study using 21 blood samples from healthy donors. Three commercial lysing solutions used in spectral FC immunophenotyping were evaluated. Samples were stained with a panel of eight antibody-conjugated fluorochromes and aliquoted for assessment with each lysing solution (Excellyse I, Excellyse Easy, and Tombo RBC Lysis Buffer), following the manufacturers' protocols. Data acquisition was performed using a full-spectrum flow cytometer.

R

SUBMITTER: Huamanyalli-Villar E 

PROVIDER: S-EPMC12853391 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature