Histone acetyltransferase KAT6A contributes to colon cancer malignant progression by inhibiting ferroptosis.

ABSTRACT:

Background

Colon cancer (CC) is a malignant cancer with high incidence and poor prognosis.Ferroptosis could induce iron-dependent accumulation of lipid peroxidation and oxidative death of cancer cells. This work aimed to elucidate the role of KAT6A, a lysine acetyltransferase, in CC development.

Methods

We collected tumor and paired non-tumor samples from patients with CC and analyzed the RNA and protein level of KAT6A using quantitative real-time PCR and immunohistochemistry (IHC) staining. We conducted KAT6A knockdown in CC cells and determined cell proliferation and ferroptosis. Cell proliferation was measured by cell counting kit-8 (CCK-8) and colony formation assay. Ferroptosis was identified by measuring the levels of lipid ROS, intracellular iron and Fe²⁺,

SUBMITTER: Huo J 

PROVIDER: S-EPMC12853672 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature