Enhanced extracellular respiration of engineered Bacillus subtilis via anodic electro-fermentation with pH optimisation.

ABSTRACT: Leveraging alternative electron acceptors to support anaerobic metabolism in industrially relevant microorganisms holds substantial biotechnological potential, especially when coupled to anodic electro-fermentation, which provides a non-depleting electron sink to the microorganisms. Bacillus subtilis is a widely used industrial workhorse for biochemical production, valued for its genetic tractability and environmental stress tolerance. However, the anaerobic, anodic metabolism of B. subtilis has been constrained by limited redox flexibility. Here, deletion of ldh (lactate dehydrogenase) to restrict fermentative NAD⁺ regeneration enabled engineered B. subtilis to survive anaerobically via anodic respiration, partially oxidising glucose while steering the metabolism toward 2,3-butanediol. Th

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12853675 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature