Fine modulation of carbon flow in central carbon metabolism via ribosome-binding site modification in <i>Escherichia coli</i>.

ABSTRACT: Optimization of flux distribution in central carbon metabolism is important to improve the microbial productivity. As the number of precursors required for synthesis differs for each target compound, optimal flux distribution also varies. A library of mutant strains with diverse flux distributions can aid in optimal strain screening. Therefore, in this study, we aimed to construct a library of Escherichia coli strains with stepwise changes in flux distribution by introducing mutations into the ribosome-binding sites of key enzyme genes on its chromosome. We focused on the flux ratios at the glucose-6-phosphate and acetyl-CoA branch points to enhance mevalonate production. Mutations were introduced into the ribosome-binding sites of pgi and gltA to vary the flux ratios

SUBMITTER: Sawada S 

PROVIDER: S-EPMC12853775 | biostudies-literature | 2026 Jun

REPOSITORIES: biostudies-literature