Adipocyte NMNAT1 expression is essential for nuclear NAD⁺ biosynthesis but dispensable for regulating thermogenesis and whole-body energy metabolism.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺) functions as an essential cofactor regulating a variety of biological processes. The purpose of the present study was to determine the role of nuclear NAD⁺ biosynthesis, mediated by nicotinamide mononucleotide adenylyltransferase 1 (NMNAT1), in thermogenesis and whole-body energy metabolism. We first evaluated the relationship between NMNAT1 expression and thermogenic activity in brown adipose tissue (BAT), a key organ for non-shivering thermogenesis. We found that reduced BAT NMNAT1expression was associated with inactivation of thermogenic gene program induced by obesity and thermoneutrality. Next, we generated and characterized adiponectin-Cre-driven adipocyte-specific Nmnat1 knockout (ANMT1KO) mice. Loss of NMNAT1 markedly r

SUBMITTER: Yamaguchi S 

PROVIDER: S-EPMC12853836 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature