Identification of the LncRNA PRNCR1/miR-642a-5p/SSTR3 CeRNA network and its diagnostic value in type 2 diabetes mellitus.

ABSTRACT:

Objective

This study aimed to investigate the expression and functions of long noncoding RNAs (lncRNAs) in circulating leukocytes related to type 2 diabetes mellitus (T2DM).

Methods

We conducted microarray analysis and RNA sequencing on peripheral blood mononuclear cells (PBMCs) from newly diagnosed T2DM patients and healthy controls. Differentially expressed lncRNAs, mRNAs, and miRNAs were identified using an absolute log₂(Fold Change) of ≥ 0.585 cutoff and a P value < 0.05. A competing endogenous RNA (ceRNA) network was constructed, and functional enrichment analyses (Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO)) were performed. Quantitative real-time PCR (qRT-PCR) was used to validate the expression of selected lncRNAs, miRNAs, and mRNA

SUBMITTER: Chen J 

PROVIDER: S-EPMC12853847 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature