Advance biocatalyst for the green conversion of 5-hydroxymethylfurfural to a biopolymer precursor 2,5-furandicarboxylic acid.

ABSTRACT:

Background

2,5-Furandicarboxylic acid (FDCA) is a bio-derived compound which serves as a building block of polyethylene furandicarboxylate (PEF) for the synthesis of bio-plastics. 5-Hydroxymethylfurfural oxidase (HMFO) is a promising biocatalyst for the oxidation of 5-hydroxymethylfurfural (HMF) to FDCA through three oxidation steps. The substrate could be fructose and other C6 sugars present in lignocellulosic biomass, providing an eco-friendly and sustainable route for the production of FDCA, an essential monomer for biopolymer development.

Result

The newly identified HMFO enzyme from Pseudomonas poae (PSPO) was heterologously expressed in E. coli, and the recombinant protein was purified and characterized. The catalytic function of PSPO was authenticated for the oxidation

SUBMITTER: Pawar L 

PROVIDER: S-EPMC12853909 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature