Effect of Enzymatic-based chemomechanical caries removal agent on proliferation and osteogenic differentiation of dental pulp stem cells.

ABSTRACT:

Background

Chemomechanical caries removal (CMCR) offers a non-invasive alternative to conventional drilling techniques; however, the potential cytotoxic and genotoxic effects of CMCR agents on the dental pulp complex remain underexplored. This study aimed to evaluate the effects of enzymatic-based CMCR on the proliferation and osteogenic differentiation of dental pulp stem cells (DPSCs).

Methods

MTT assay, scratch assay, and gene expression analysis were conducted to assess cell proliferation (viability percentage), migration (wound healing percentage), and osteogenic differentiation (RT-qPCR). The viability of DPSCs after direct exposure to enzymatic-based CMCR (0.5%, 1%, and 2% in DMEM) was measured at 24 and 48 h post-exposure. Indirect exposure was also tested using soun

SUBMITTER: Gouda HA 

PROVIDER: S-EPMC12853919 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature