Monocyte-Derived Macrophages-Synovial Fibroblasts Crosstalk Unravels Oncostatin Signaling Network as a Driver of Synovitis in Osteoarthritis.

ABSTRACT:

Objective

Osteoarthritis (OA) is a debilitating joint disease that is characterized by cartilage degradation, synovial inflammation, and pain. Macrophages have been implicated in OA pathology, but the origins and functions of diverse macrophage subsets seeding the synovial joint tissue remain incompletely understood. This study investigates macrophage heterogeneity, ontogeny, fate, and communication with stromal niche cells in OA.

Methods

Single-cell RNA sequencing was employed on synovial cells isolated from mice with collagenase-induced OA (CiOA) and CD14⁺ macrophages from four patients with OA. We combined flow cytometry, genetic fate mapping, and imaging mass cytometry to profile synovial macrophage subsets. Cell-cell communication analyses were performed to i

SUBMITTER: Laouteouet D 

PROVIDER: S-EPMC12854009 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature