A pbpB1 mutation causing reduced β-lactam susceptibility in clinical Listeria monocytogenes isolates.

ABSTRACT: Listeriosis is a severe foodborne infection and associated with high mortality. Treatment is based on ampicillin, amoxicillin or penicillin, often combined with gentamicin, but meropenem is also used occasionally. β-lactam resistant Listeria monocytogenes isolates are infrequently described but the mechanism of resistance is not known. A clinical L. monocytogenes isolate with reduced β-lactam susceptibility was collected from a German listeriosis patient. Resistance profiling, whole genome sequencing, comparative genomics and genetic experiments were used to identify the causative DNA polymorphism. A W428R substitution near the active site of penicillin binding protein B1 (PBPB1) was identified as the cause of reduced ampicillin, amoxicillin and meropenem susceptibility. Furt

SUBMITTER: Wamp S 

PROVIDER: S-EPMC12854224 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature