Longitudinal In Vitro Assessment of the Optical Transparency of Preserved Pre-Descemet Layer in the Absence of Corneal Endothelium.

ABSTRACT:

Purpose

The purpose of this study was to evaluate the optical transparency morphological and ultrastructural features of the isolated pre-Descemet layer (PDL) in the absence of endothelium after preservation for up to 12 months using standard organ culture and silica gel-based tissue dehydration.

Methods

Following removal of the Descemet membrane endothelium complex, the PDL was isolated using the big bubble technique. Ten samples were preserved either in standard organ culture medium (group 1) or by silica gel-based dehydration (group 2). Five samples with the PDL with thin posterior stroma served as the controls (group 3). Transparency was quantified using a custom-built optical device, and thickness was measured by anterior segment optical coherence tomography at baseline

SUBMITTER: Busin M 

PROVIDER: S-EPMC12854240 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature