Effect of Repeated Medium-Dose Red Laser Irradiation on Ocular Tissues in Guinea Pigs.

ABSTRACT:

Purpose

The purpose of this study was to investigate the effects of repeated red laser irradiation at a supraclinical dose on histology and apoptosis-related molecular markers in guinea pig ocular tissues.

Methods

Guinea pigs (1 month old) were randomized into two groups: the repeated irradiation group and the control group. After pupil dilation, the repeated irradiation group received binocular red laser (650 nm, 10 mW) exposure for 3 minutes per session, 6 sessions daily, over 8 days. Ocular surface morphology was examined using slit lamp microscopy. Retinal histopathology was assessed by hematoxylin and eosin (H&E) staining, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, immunohistochemistry, and immunofluorescence. Protein and mRNA expression

SUBMITTER: Song QQ 

PROVIDER: S-EPMC12854241 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature