ABSTRACT: Anthrax, or charbon, is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis, responsible for sporadic outbreaks and representing a significant public health issue in Peru. In this study, 18 isolates from humans, animals and soil, collected between 2005 and 2017 across different country regions, were reactivated and analyzed. We identified antimicrobial resistance genes, virulence factors, plasmids, and their phylogenetic relationships using next-generation sequencing and bioinformatics tools. We detected eight genes associated with multidrug resistance, including vanZ-F, mphL, fosB_gen, fosB, satA, bla, bla2, and fosB2. Additionally, 24 chromosomal virulence genes were identified, related to toxin production, capsule biosynthesis, secretion systems, and iron acquisi
