Detergent-Triggered Membrane Remodelling Monitored via Intramembrane Fluorescence Dequenching.

ABSTRACT: Detergent-induced membrane solubilization is important for several biotechnological applications including membrane protein isolation, cell lysis and virus inactivation. The thermodynamic details of the underlying process have been previously examined, but the mechanistic details remain largely underexplored owing in part to a lack of suitable technologies capable of assessing nanoscopic membrane disruption events. Key open questions include: how do detergents remodel the membrane structure at subsolubilizing concentrations? And what is the sequence of morphological transitions that lead up to solubilization? Here, we introduce a single-color assay based on the fluorescence dequenching of membrane-integrated fluorophores as a sensitive and generalizable tool to probe nanoscale membrane rem

SUBMITTER: Andrews CMF 

PROVIDER: S-EPMC12854627 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature