Combinatorial Synthesis of Protein-Polymer Conjugates by Postpolymerization Modification of Poly(pentafluorophenyl acrylate)s.

ABSTRACT: Combinatorial methods for preparing polymeric biomaterials enable the rapid identification of materials useful for many applications in science, medicine, and engineering. In the work described here, we demonstrate that side-chain reactive polymers can be used as templates for the rapid preparation of a small library of diversely functionalized protein-polymer conjugates. The activated ester polymer poly-(pentafluorophenyl acrylate) (PPFPA) was modified postpolymerization with substoichiometric equivalents of three hydrophilic primary amines to yield a library of amphiphilic, side-chain reactive copolymers. These copolymers were then conjugated to two receptor-targeting proteins, holotransferrin (hTF) and an engineered fibronectin-based protein (Fn3), through amine-activated ester coupling

SUBMITTER: Kish EW 

PROVIDER: S-EPMC12854635 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature