Confocal Raman Microscopy Quantitatively Reveals Subcellular Metabolic Adaptation Mechanisms of Deep-Sea Mussels to Methane Deprivation.

ABSTRACT: Methane hydrates are a potential energy source, but their extraction may disrupt deep-sea ecosystems that rely on methane. Understanding biological responses to methane fluctuations in cold seep environments remains limited, primarily due to the inability to monitor subcellular metabolic dynamics in real time. This study applied confocal Raman microscopy combined with full-spectrum normalization to investigate subcellular metabolic adaptations in the gill tissues of the deep-sea mussel containing symbionts under methane deficiency. This label-free and nondestructive technique allows high-resolution quantitative analysis of biomolecules. Results demonstrate that methane deficiency induced metabolic reprogramming and structural reorganization, disrupting energy metabolism while enhancing pro

SUBMITTER: He W 

PROVIDER: S-EPMC12854740 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature