Targeting TatD nuclease and the MAPK Pathway: Luteolin multifaceted approach against Mycoplasma gallisepticum infection.

ABSTRACT: Nucleases are considered to be a key virulence factor of Mycoplasma gallisepticum (MG) and targeting nucleases is considered a novelly and potential direction for developing anti-MG drugs. The TatD nuclease localizes to the MG cell cytoplasm and can be released via extracellular vesicles (EVs), enabling it to participate in the immune evasion process. In this study, we explore the pathogenic mechanisms of TatD nuclease and screens for potential inhibitors, which could help overcome the limitations of MG treatment. In order to observe the function of TatD nuclease within cells, We utilized the transmembrane peptide Trans-activator (TAT) to construct the Trans-activator-TatD (TAT-TatD) recombinant protein. The effects of TAT-TatD recombinant protein were evaluated using DNA hydrolysis assays

SUBMITTER: Liu W 

PROVIDER: S-EPMC12854987 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature