Harnessing mRNA for the expression of monoclonal IgG and IgA in non-human primates.

ABSTRACT:

Background

Monoclonal antibodies (mAbs) are an increasingly essential class of medicines across many disease areas. In the human body, there are five antibody isotypes, each with potential therapeutic benefits for different disease indications. However, 97% of all clinically approved mAbs are produced as the IgG isotype, largely due to challenges associated with recombinantly producing non-IgG isotypes like IgM or IgA, which have additional N-linked glycan sites and can present as multivalent oligomers. One potential way to circumvent this challenge is to express mAbs in situ using mRNA encapsulated in lipid nanoparticles (LNP), bypassing the need for recombinant protein production.

Objective

Here, we demonstrate the feasibility of expressing a mAb as both IgG and IgA in non

SUBMITTER: Rouzeau R 

PROVIDER: S-EPMC12855044 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature