Liu Shen Wan regulates the SPHK1/S1P axis to ameliorate influenza-induced inflammation via integrated network pharmacology and lipidomics.

ABSTRACT:

Background

Liu Shen Wan (LSW) can modulate sphingolipid metabolism, which is a key pathway in inflammatory regulation, yet the precise mechanistic actions remain elusive. This study aimed to elucidate the mechanism by which LSW regulates sphingolipid metabolism to mitigate influenza-induced inflammatory responses.

Methods

The potential mechanisms of LSW were initially predicted and validated via network pharmacology and lipidomics. A549 cells were infected with influenza A/Puerto Rico/8/34 (H1N1) (PR8) or transfected to overexpress sphingosine kinase-1 (SPHK1), then treated with LSW. In vivo, mice were infected with PR8 or challenged with rAAV9-SPHK1 and administered LSW for 5 days. Inflammatory factors and sphingolipid pathway-associated proteins were evaluated.

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SUBMITTER: Lei B 

PROVIDER: S-EPMC12855086 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature