Stem cell specification and niche formation in developing incisor require actomyosin forces.

ABSTRACT:

Background

The precise timing of stem cell specification and niche formation during murine incisor development is poorly understood, and it is unclear whether these processes occur simultaneously or in a sequential manner. Functional dental epithelial stem cells are marked by the expression of Sox2, a transcription factor that is broadly expressed in the dental epithelium at the dentition onset and restricted to stem cells in fully developed incisor.

Methods

Using genetic lineage tracing in Sox2CreERT2/+; R26RmT/mG and Sox2CreERT2/+; R26RtdT/+ embryos along with a single-cell RNA sequencing at different stages of incisor development, we investigated the timing of the stem cell specification and its temporal relationship with niche formation.

Results

Our results reveal

SUBMITTER: Mohtadi Hamadani Y 

PROVIDER: S-EPMC12855154 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature