Development of a recombinase-aided isothermal amplification method coupled with a lateral flow dipstick assay for the diagnosis of powdery scab in potatoes.

ABSTRACT: Potato powdery scab is a soilborne disease caused by the fungus Spongospora subterranea, which belongs to the class of Plasmodiophorids and cannot be cultured. In this study, a species-specific genomic DNA fragment of Spongospora subterranea (2494 bp) was identified using comparative genomics methods. Polymerase chain reaction (PCR) and recombinase-aided amplification-lateral flow dipstick (RAA-LFD) base assays were then developed for the specific detection of this pathogen. Both detection methods effectively distinguished Spongospora subterranea from other common potato pathogens, and Polymyxa graminis and Plasmodiophora brassicae, the primary pathogens of the intercropping cruciferous and gramineous plants. The detection sensitivity of the three PCR pri

SUBMITTER: Yang J 

PROVIDER: S-EPMC12855457 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature