A transmission relationship investigation of HIV infection through male-to-male sex among a case of left-behind children with heterosexual orientation in Zhejiang Province of China.

ABSTRACT:

Objective

This study seeks to trace the source of HIV infection in a male adolescent (P1) with a newly recognized heterosexual orientation in Zhejiang Province on August 23, 2019.

Methods

An in-depth epidemiological interview was conducted with P1 and all sexual partners. For HIV-infected partners, sequential partner tracing was performed until no new HIV cases were detected. The survey assessed high-risk sexual behavior, HIV testing and treatment history, and demographic details. Serum samples were collected for HIV antibody testing, and plasma samples from HIV-positive individuals were taken before antiviral therapy. HIV DNA was extracted, and specific gene regions were amplified, cloned, and sequenced. Phylogenetic trees were created using MEGA V6.0 to identify HIV subtyp

SUBMITTER: Hou Z 

PROVIDER: S-EPMC12855501 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature