Modifying the upstream open reading frames of cellulase gene enhances cellulase production in <i>Penicillium oxalicum</i>.

ABSTRACT: Cellulase plays an irreplaceable role in biomanufacturing using plant biomass as feedstock. However, improving cellulase production by fungi through manipulation of upstream open reading frames (uORFs) in the 5'-untranslated regions (5'-UTR) of cellulase genes has been less frequently explored. This study aimed to screen uORFs in the 5'-UTR of cellulase genes in Penicillium oxalicum, identify functional uORFs in the 5'-UTR of the eg1 gene which encodes a key endo-β-1,4-glucanase (EG) in P. oxalicum, and enhance fungal cellulase production through uORF modifications. Among the 25 cellulase genes examined in P. oxalicum strain HP7-1, 23 contained uORFs in their 5'-UTR. Seven uORFs were annotated in the 5'-UTR of the eg1 gene. A uORF-green fluo

SUBMITTER: Zhao S 

PROVIDER: S-EPMC12855567 | biostudies-literature | 2026 Jun

REPOSITORIES: biostudies-literature