Development and validation of a quadruplex qPCR assay for simultaneous detection of GPV, GoCV, TMUV, and GAstV-Ⅱ in geese.

ABSTRACT: Co-infections with multiple viruses are common in geese, complicating clinical diagnosis. Here, we developed a quadruplex TaqMan-probe based qPCR method for the simultaneous detection of Goose parvovirus (GPV), Goose circovirus (GoCV), Tembusu virus (TMUV), and Goose astrovirus Ⅱ (GAstV-Ⅱ). Specific primers and hydrolysis probes were designed targeting the following conserved regions: the VP3 gene of GPV, the Rep gene of GoCV, the E gene of TMUV, and the ORF2 gene of GAstV-Ⅱ, respectively. No cross-reactivity was observed with other common avian pathogens, including Goose paramyxovirus (GPMV), Fowl adenovirus (FADV), Newcastle disease virus (NDV, genotype VII ), Escherichia coli (E. coli), Pasteurella multocida (P. multocida). The limits of detection (LOD) for GPV, GoCV, TMUV, and GAstV-Ⅱ

SUBMITTER: Li M 

PROVIDER: S-EPMC12855954 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature