Assessing the Performance of Mass Spectrometry Search Strategies in Identifying Translational Errors Using PDX Proteomics Data.

ABSTRACT: Translational errors (TEs) result in a mismatch between mRNA codons and the amino acids (AAs) of the corresponding protein. Unlike DNA mutations or RNA editing, where nucleotide sequences can be used to infer AA substitutions, TEs can only be detected at the protein level. Although high-throughput mass spectrometry (MS) proteomics offers the potential to resolve peptide sequences and could theoretically be used to identify TEs, the feasibility of current MS data analysis approaches for this application remains uncertain. Here, we utilize patient-derived xenograft proteomics data, which include both human and mouse peptides with identifiable cross-species AA variations, as a ground truth for benchmarking TE identification methods. By using high-confidence mouse peptides as surrogates for "T

SUBMITTER: Mahmud A 

PROVIDER: S-EPMC12856148 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature